복분자 추출물의 Candida albicans 성장억제 효과
Growth inhibition effect of Rubus coreanus Miquel on Candida albican
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신애리:Shin Ae-Ri
옥승호:Ohk Seung-Ho/최충호:Choi Choong-Ho/홍석진:Hong Suk-Jin
KMID : 0355420150390030168
Abstract
Objectives: In this study, the growth inhibition effect of Rubus coreanus Miquel on Candida albicans (C. albicans) was observed.
Methods: The Rubus coreanus Miquel was extracted with 70% methanol and concentrated with a rotary evaporator. Antifungal effect of Rubus coreanus Miquel extract on C. albicans was determined by paper disc diffusion method and standard plate count method. Seven different concentrations (2, 4, 8, 15, 30, 60, 120 mg/ml) of the extract were tested by paper disc diffusion method. Two kinds of concentration (8, 60 mg/ml) of the extract were tested using standard plate count method on C. albicans with different incubating time (for 6, 12, 24 hours immediately after the cultivation). Morphological changes of C. albicans cells after exposure to the extract were observed with scanning electron microscopy (SEM) images.
Results: The Rubus coreanus Miquel extract showed an antifungal effect on C. albicans in 8, 15, 30, 60, 120 mg/ml of concentrations (P<0.05). The extract with 8 mg/ml of concentration showed about 30% of growth inhibition at 6 h and with 60 mg/ml it showed about 90% of growth inhibition at 24 h. SEM analysis showed damaged surfaces of C. albicans cells when treated with Rubus coreanus Miquel extract.
Conclusions: The Rubus coreanus Miquel might have the potential as a nobel growth inhibitory agent against C. albicans that causes oral infection.
키워드
Candida albicans; Growth inhibition effect; Rubus coreanus Miquel
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